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Journal: Cell Genomics
Article Title: Systematic cell-type resolved transcriptomes of 8 tissues in 8 lab and wild-derived mouse strains capture global and local expression variation
doi: 10.1016/j.xgen.2025.101108
Figure Lengend Snippet: Overview of the IGVF mouse dataset in eight founder genotypes (A) Visual representation of the “8-cube” dataset across eight genotypes, eight tissues, and eight replicates. (B) Experimental design of sample barcoding plate. Nuclei derived from one tissue serves as the main tissue on the plate. Nuclei from a different tissue are multiplexed in the remaining third of the plate, where two samples from distinct genotypes are loaded into one well. See also , , ; and . (C) Tissue-loading pattern for all eight sample barcoding plates. (D) Pseudobulk PCA of 516 total samples colored by tissue and points indicating females (crosses) and males (circles). See for PCA per tissue. (E) Adjusted p values from two-sided ANOVA test between PCs and metadata (top heatmap); scores for the first nine PCs across samples (bottom heatmap). (F) Row-scaled expression of 20 protein-coding genotype-specific genes across pseudobulk samples grouped by tissue, genotype, and sex. See for full gene list. (G) Number of differentially expressed genes (DEGs) between each genotype and B6 ( n = 8 samples per group, gonads n = 4). Two-sided Wald test, Benjamini-Hochberg adjusted p value <0.01 and absolute log fold change (LFC) >1; see also ). (H) Percentage of nuclei falling into genotype-specific subclusters across tissues (see also ).
Article Snippet:
Techniques: Derivative Assay, Expressing